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. Author manuscript; available in PMC: 2021 Oct 27.
Published in final edited form as: Biochemistry. 2020 Oct 15;59(42):4131–4142. doi: 10.1021/acs.biochem.0c00672

Figure 1∣. Endogenous NBDY quantitation and interaction with the human cytoplasmic RNA decapping complex.

Figure 1∣

(a) Quantification of endogenous protein levels (NBDY and EDC4) in HEK 293T cells by Western blotting. (b) Endogenous NBDY co-immunopurified with FLAG-EDC4 (top) and FLAG-DCP1A (bottom) stably expressed in HEK 293T cells. WT, wild type; KO, NBDY knock out.