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. Author manuscript; available in PMC: 2021 Oct 27.
Published in final edited form as: Biochemistry. 2020 Oct 15;59(42):4131–4142. doi: 10.1021/acs.biochem.0c00672

Figure 4∣. Low-micromolar association of NBDY with EDC4 in vitro requires residue W34 of NBDY.

Figure 4∣

(a) Surface plasmon resonance response for purified NBDY binding to the purified EDC4 WD40 domain. (b) Affinity and specificity measurement of EDC4 WD40 domain binding to NBDY (left) or the non-interacting W34A NBDY mutant (right) by isothermal titration calorimetry. From low to high, curves represent EDC4 concentrations increasing from 0.025 μM to 5 μM.