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. Author manuscript; available in PMC: 2021 Oct 27.
Published in final edited form as: Biochemistry. 2020 Oct 15;59(42):4131–4142. doi: 10.1021/acs.biochem.0c00672

Figure 7∣. The NBDY-EDC4 interaction is required for RNA half-life specification of transcripts co-regulated by NBDY and DCP2.

Figure 7∣

(a,b) NBDY localization and P-body numbers were assayed in NBDY and NBDY W34A rescue cell lines. Quantitative data (right) represents mean values ± s.e.m, and significance was evaluated with one-way ANOVA. **P < 0.01, Dunnett’s test). Scale bars, 20 μm. (c) RNA stability measurement for selected genes in NBDY rescue versus NBDY W34A (non-EDC4-interacting mutant) complementation cell line. Selected genes fall into the following classes: stabilized in both DCP2 KO and NBDY KO (EPC2), stabilized by DCP2 KO but destabilized in NBDY KO (GJC1), destabilized in both DCP2 KO and NBDY KO (ATM), and exclusively stabilized in NBDY KO (ZNF84). Number of biological replicates: n=3 for EPC2 and n=4 for all other genes. Error bars represent mean ± s.d. **P < 0.01, linear regression t-test.