Fig. 6.

a Schematic view of the final vaccine construct. The T-cell and B-cell epitopes were included in the vaccine construct, and they were linked via AAY linker (L). A cysteine residue was added at the N-terminal of the vaccine required for conjugation with a carrier molecule. The construct is 33 amino acid long, and that is non-allergic in nature predicted by the AllerTop v.2 and AlgPred server. b Secondary structure properties of the vaccine model by SOPMA. c 3D model of the final vaccine constructs, i. the preliminary form generated by iTASSER, ii. Refined by Galaxy refine server, and iii. Merged. d Validation of the structure using PROSA with a Z score of − 0.14