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. 2020 Nov 18;13:11883–11897. doi: 10.2147/OTT.S284192

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© 2020 Lu et al.

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Hsa-circ-0048117 promotes M2 polarization by inhibiting miR-140. (A) The 10 candidate miRNAs binding to hsa-circ-0048117 were listed according to RNA Interactome and miRanda miRNA target prediction tools. (B) CircRIP was used to purify the hsa-circ-0048117 related miRNAs. (C) AGO2 antibody for RNA immunoprecipitation was used to verify hsa-circ-0048117 could be enriched by AGO2 antibody (****p<0.001). (D) Pull-down experiment of the biotin-coupled miR-140 mimic was conducted to find hsa-circ-0048117 was significantly enriched in macrophages transfected with miR-140 mimics (****p<0.001). (E) The luciferase reporter experiment was applied to detect the luciferase activity after miR-140 mimics binding to hsa-circ-0048117 (****p<0.001). (F) FISH detection showed that hsa-circ-0048117 and miR-140 were co-localized in macrophages. (G) The predicted binding sequence between miR-140 and hsa-circ-0048117. (H and I) Flow cytometry was used to detect the M2 markers CD14 and CD206 to check macrophages polarization under the joint effect of miR-140 and hsa-circ-0048117 (****p<0.001).