Table 2. The primers used for a quantitative real-time polymerase chain reaction.
*) the data for other twenty HDL-related and three housekeeping genes were published [14]
| Primer | GeneBank | 5’- 3’ nucleotide sequence | location | efficiency |
|---|---|---|---|---|
| Genes involved in atherogenesis | ||||
| ASGR2 (F) | NM_001181.4 | GGAGAAACAGCAGCAGGACC | 663–682 | 1.65 |
| ASGR2 (R) | GAGTGAGAGAACCAGTAGCAGC | 827–848 | ||
| CD14 (F) | NM_000591.3 | CAAGTGTGAAGCCTGGAAGC | 277–296 | 1.80 |
| CD14 (R) | ACAAGGTTCTGGCGTGGTC | 436–454 | ||
| CD36 (F) | NM_000072.3 | CCTTTGCCTCTCCAGTTGAA | 1310–1329 | 1.99 |
| CD36 (R) | GTACACAGGTCTCCCTTCTTTG | 1413–1434 | ||
| CSF1R (F) | NM_005211.3 | GGTGGCAGGAAGGTGATGT | 836–854 | 1.71 |
| CSF1R (R) | GGTGTTGTTGTGTTGGAGGA | 999–1018 | ||
| CSF2RB (F) | NM_000395.2 | ATCCTCCTCTCCAACACCTCC | 776–796 | 1.80 |
| CSF2RB (R) | ACCTCCTTCCTCACCTCCCA | 1001–1020 | ||
| CXCL5 (F) | NM_002994.4 | GCTGTTGGTGCTGCTGCT | 208–225 | 1.81 |
| CXCL5 (R) | CCGTTCTTCAGGGAGGCTAC | 389–408 | ||
| CYBA (F) | NM_000101.3 | TTGTGTGCCTGCTGGAGTA | 214–232 | 1.70 |
| CYBA (R) | AGTAGGTAGATGCCGCTCG | 421–439 | ||
| F5 (F) | NM_000130.4 | CTGGCTGGTGGCTCCTAA | 5781–5798 | 1.57 |
| F5 (R) | ATCTTGCTAATCTGGGCTCC | 5941–5960 | ||
| JAM3 (F) | NM_032801.4 | TTCCAGAGCCAATCCCAGA | 708–726 | 1.90 |
| JAM3 (R) | TCCGCCAATGTTCAGGTC | 874–891 | ||
| ICAM1 (F) | NM_000201.2 | GTGACCGTGAATGTGCTCTCC | 1727–1747 | 1.83 |
| ICAM1 (R) | GAGGCGTGGCTTGTGTGTT | 1895–1913 | ||
| IL1B (F) | NM_000576.2 | TGTCCTGCGTGTTGAAAGATGA | 641–662 | 1.84 |
| IL1B (R) | CTGCTTGAGAGGTGCTGATGTA | 796–817 | ||
| IL1R1 (F) | NM_000877.3 | TGCTTACTGGAAGTGGAATG | 1099–1118 | 1.76 |
| IL1R1 (R) | TGCTGCATCTATACCATGTG | 1278–1297 | ||
| IL18 (F) | NM_001562.3 | GACCAAGGAAATCGGCCT | 395–412 | 1.81 |
| IL18 (R) | CACAGAGATAGTTACAGCCATACC | 503–526 | ||
| IL18RAP (F) | NM_003853.3 | ACAACCCAGTCCGTCCAAC | 1524–1542 | 1.86 |
| IL18RAP (R) | ACATCAGGAAATAGGCTCAGG | 1790–1810 | ||
| IL18R1 (F) | NM_003855.3 | CGATAAAGAAGAACGCCGAGT | 655–675 | 1.68 |
| IL18R1 (R) | GCAGAGCAGTTGAGCCTTACG | 842–862 | ||
| ITGAM (F) | NM_001145808.1 | CTCTCTCCCAGGCTCCAGT | 1827–1845 | 1.79 |
| ITGAM (R) | CATTCCTTGCCACTTCCCT | 1986–2004 | ||
| ITGA2B (F) | NM_000419.4 | AAGATTGTGCTGCTGGACG | 2421–2439 | 1.81 |
| ITGA2B (R) | GAAGGTGGATGCTGAGGTGA | 2612–2631 | ||
| ITGB3 (F) | NM_000212.2 | AGTAACCTGCGGATTGGCTT | 537–556 | 1.81 |
| ITGB3 (R) | CACACTCTGCTTCTTCACTTCC | 713–734 | ||
| LTA (F) | NM_001159740.2 | GCTGCTGCTGGTTCTGCT | 282–299 | 1.65 |
| LTA (R) | GTTCTGCTTGCTGGGGTCT | 420–438 | ||
| MGST1 (F) | NM_145792.2 | CAGGTAATGGATGATGAAGTA | 81–101 | 1.79 |
| MGST1 (R) | GCCAAATGCTACACAGTCTTCT | 206–227 | ||
| MMP9 (F) | NM_004994.2 | ACCCTTGTGCTCTTCCCTG | 98–116 | 1.70 |
| MMP9 (R) | CGACTCTCCACGCATCTCTG | 192–211 | ||
| NPC1 (F) | NM_000271.4 | CAGCCACATAACCAGAGCGT | 3778–3797 | 1.78 |
| NPC1 (R) | AGCCAACACCACAATCCCT | 3898–3916 | ||
| NPC2 (F) | NM_006432.3 | TCCCATTCCTGAGCCTGAT | 362–380 | 1.82 |
| NPC2 (R) | GTTGCCACTCCACCACCA | 478–495 | ||
| NR1H2 (F) | NM_007121.5 | GCCATCATCTCAGTCCAGG | 1104–1122 | 1.79 |
| NR1H2 (R) | ACTCTGTCTCGTGGTTGTAGC | 1237–1257 | ||
| NR1H3 (F) | NM_005693.3 | GCCTTGCTCATTGCTATCAG | 1319–1338 | 1.86 |
| NR1H3 (R) | GTGGGAACATCAGTCGGTCA | 1447–1466 | ||
| OLR1 (F) | NM_002543.3 | TTGCCTGGGATTAGTAGTGACC | 249–270 | 1.77 |
| OLR1 (R) | CTTCTTCTGCTTGTTGCCG | 376–394 | ||
| PCTP (F) | NM_021213.3 | GGTGAAGCAATACAAGCAGAG | 578–598 | 2.18 |
| PCTP (R) | TAATGAGCCAGGACGGAAT | 672–690 | ||
| PLA2G7 (F) | NM_005084.3 | GGCATTGACCTGGCATCTC | 759–777 | 1.86 |
| PLA2G7 (R) | TGTGTCTCCTCCTCTTGTTTCAG | 894–916 | ||
| PRKCQ (F) | NM_006257.4 | ACTGCCACCTTCTTCCCAC | 587–605 | 1.55 |
| PRKCQ (R) | CTTGAGTCCTTGCCGTGC | 866–883 | ||
| SLC7A11 (F) | NM_014331.3 | GTCCGCAAGCACACTCCT | 1358–1375 | 1.97 |
| SLC7A11 (R) | ATGACGAAGCCAATCCCTG | 1629–1647 | ||
| SLPI (F) | NM_003064.3 | CCTTCCTGGTGCTGCTTG | 44–61 | 2.28 |
| SLPI (R) | GACAACATCTCTTCTTCCCTGG | 193–214 | ||
| SOD2 (F) | NM_000636.3 | CACCACAGCAAGCACCAC | 309–326 | 1.80 |
| SOD2 (R) | GTTCTCCACCACCGTTAGG | 480–498 | ||
| SREBF1 (F) | NM_001005291.2 | CCTCAGATACCACCAGCGTC | 1828–1847 | 1.68 |
| SREBF1 (R) | TTGCGATGCCTCCAGAAGT | 2026–2044 | ||
| S100A8 (F) | NM_001319196.1 | ATGCCGTCTACAGGGATGA | 267–285 | 1.74 |
| S100A8 (R) | ACGCCCATCTTTATCACCAG | 407–426 | ||
| S100A9 (F) | NM_002965.3 | GACCATCATCAACACCTTCCAC | 82–103 | 1.68 |
| S100A9 (R) | TAGCCTCGCCATCAGCAT | 284–301 | ||
| S100A12 (F) | NM_005621.1 | ATTAGGCTGGGAAGATGACAA | 55–75 | 1.90 |
| S100A12 (R) | GTGGGTGTGGTAATGGGCA | 320–338 | ||
| TLR5 (F) | NM_003268.5 | GACCCTCTGCCCCTAGAATAA | 581–601 | 1.75 |
| TLR5 (R) | GCCATCAAAGGAGCAGGAA | 707–725 | ||
| TLR8 (F) | NM_016610.3 | GGAACATCAGCAAGACCCATC | 115–135 | 1.80 |
| TLR8 (R) | CGCATAACTCACAGGAACCAGA | 279–300 | ||
| TNFRSF1A (F) | NM_001065.3 | GCTCCTTCACCGCTTCAGA | 563–581 | 1.74 |
| TNFRSF1A (R) | GGTCCCATTGAGGCAGAGG | 744–762 | ||
| TNFRSF1B (F) | NM_001066.2 | AACACACGCAGCCAACTCC | 763–781 | 1.77 |
| TNFRSF1B (R) | GTCACACCCACAATCAGTCCA | 875–895 | ||
| VEGFA (F) | NM_001025366.2 | GAGGGCAGAATCATCACGAA | 1136–1155 | 1.79 |
| VEGFA (R) | CATCAGGGGCACACAGGA | 1264 – 1281 | ||
| HDL-related genes*) | ||||
| LDLR (F) | NM_000527.4 | GAGGTGGCCAGCAATAGAA | 1490–1508 | 2.00 |
| LDLR (R) | GATGACGGTGTCATAGGAAGAG | 1572–1593 | ||
| LIPC (F) | NM_000236.2 | CTTCAACTCCTCCCTGCCTCT | 285–305 | 1.87 |
| LIPC (R) | TGGTGTAGTGGTCGTGGGC | 433–451 | ||