Table 1. Tubulin missense mutants isolated in this study.

Strain	Parent strain	Gene altered	Mutation (DNA)	Mutation (protein)	Growth	Resistance to
						propyzamide	oryzalin	colchicined
tub2-A					normalc	+/-	+/-	+/-
tua1-A x tub1-B					normalc	+/-	+/-	+/-
tua2-A x tub1-B					normalc	+/-	+/-	+/-
ory2	tua2-A x tub1-B	tua1	TAC->CACa1	Y24Ha1	normal	-	++	+/-
ory3			TTC->CTC	F52L	normal	+	++	+/-
ory4			TCC->GCC	S165A	normal	+/-	++	+/-
ory5					normal	+/-	++	+/-
pyz9	tub2-A		TTC->TTA	F351L	normal	+	+/-	-
ory6	tua1-A x tub1-B (E2)	tua2	TAC->AAC	Y24N	normal	+/-	+	+/-
ory7			TTC->TGC	F49C	normal	+/-	+	+/-
ory8			TTC->GTC	F138V	slow	+/-	++	+/-
pyz10	tub2-A	tub1	GAG->AAG	E198K	normal	++	+/-	+/-
pyz11					normal	++	+	+/-
pyz12			TTC->TGC	F266C	normal	++	+/-	+
pyz13					normal	++	+/-	+
pyz14					normal	++	+/-	+
pyz2	tua2-A x tub1-B	tub2	CAG->CAT	Q134H	normal	+	+/-	+/-
pyz3					slow	+	+/-	-
pyz4			GAG->AAG	E198K	normal	++	+	+
pyz5			ATC->AAT	I236Nb	normal	++	+	+
pyz6			AAG->GAGa2	K350Ea2	slow	++	+	+
pyz7			ATC->TTC	I368F	normal	++	+/-	-
pyz8					normal	++	+/-	-

++, highly resistant; +, resistant; +/-, parent-strain level; -, hypersensitive. For criteria, see text.

^a1Mutation previously reported for upA12 [3].

^a2Mutation previously reported for col^R4 [6].

^bMutation found in human cancer cells resistant to 2-methoxyestradiol [36].

^cCompared with a wild type (CC-124).

^dAssessed by visual inspection of 7-d cultures on agar plates containing 0–4 mM colchicine.