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. 2020 Nov 23;15(11):e0242593. doi: 10.1371/journal.pone.0242593

Fig 2. Binding of rBucl8-derived constructs to extracellular matrix proteins.

Fig 2

(A) Screening assay for rBucl8-CL-Ct binding to extracellular matrix proteins. Ligand binding was tested by ELISA; representative examples of rBucl8-CL-Ct-binding-positive and binding-negative ligands are shown. rBucl8-CL-Ct binding was compared statistically with binding to BSA-coated wells plus two standard deviations; Student’s t-test, **p ≤ 0.01. (B) Concentration-dependent binding of rBucl8-CL-Ct and rBucl8-Ct to fibrinogen. Wells were coated with fibrinogen and either recombinant Bucl8-derived protein was added at increasing concentrations. Data represents the mean ±SEM of three independent experiments (n = 3), each performed in triplicate wells. Binding was detected with an anti-His-tag mAb.