Fig 3. Histological characterization of fetal wound tissue depicted the re-epithelialization and vascularization of healing tissue.

(a) Microscopic image of hematoxylin and eosin (H&E) stained fetal wound tissue obtained at 48h post-wounding (at E17.5 post embryo formation), wound site is marked by dotted line and wound edge is marked by arrows (upper panel). Scale bar = 200 μm. Zoomed images from the wound edge and wound bed (lower panel). Scale bar = 20 μm. (b) Immunohistochemical staining of keratinocytes (Keratin 14, green) and nucleus of cell (DAPI, blue) of the fetal wound tissue obtained at 12 h and 48h post-wounding (upper panel), (Scale bar = 100 μm). Zoomed images (Scale bar = 20 μm). The break in the epithelium at 12 h panel and hyperproliferative epithelium at 48 h panel represented the site of wounding respectively.