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. 2020 Nov 23;15(11):e0242592. doi: 10.1371/journal.pone.0242592

Fig 3. Improved affinity between FP1-10 and FP11 after engineering in mammalian cells.

Fig 3

A-D, flow cytometry analysis of cell fluorescence by transient transfection in HEK293T expressing A. full length mNG2, B. mNG21-10/11, C. mNG3A1-10/11, and D. mNG3K1-10/11. E-F flow cytometry analysis of cell fluorescence by transient transfection in HEK293T expressing E. CloGFP01-10/11 and F. CloGFP1-10/11. The data are normalized to a full-length control.