Fig 4. Significant signal amplification by tandem mNG2₁₁ with mNG3A_1-10 and mNG3K_1-10.

A-C. Fluorescence intensity distribution of individual cells transiently co-transfected with FP₁₁ (1x mNG2₁₁ in green or 5x mNG2₁₁ in pink) and FP_1-10 (mNG2_1-10, mNG3A_1-10 or mNG3K_1-10), each condition has four replicates, solid lines show the mean of four distributions, the shadow indicates standard deviation. Representative images of the cells are shown in S4 and S5 Figs in S1 File. D. Each marker shows the median nuclear intensity for a field of view represented in A-C. Bars indicate the mean of all fields. Increased signal for mNG3A and mNG3K using 5x mNG2₁₁ is statistically significant (Welch’s t-test).