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. 2020 Nov 6;9:e54835. doi: 10.7554/eLife.54835

Figure 4. Excitatory hippocampal input to prefrontal fast-spiking interneurons (FSINs) is reduced in Pogz mutants.

(A, B) Representative examples of optically-evoked excitatory post-synaptic currents (oEPSCs) recorded from prefrontal FSINs in wildtype (A) or Pogz+/- mice (B). (C, D) Representative traces of optically-evoked excitatory post-synaptic potentials (oEPSPs) and action potentials recorded from FSINs in wildtype (C) or Pogz+/- mice (D). (E) The total oEPSC charge in FSINs is reduced in Pogz+/- mice, U = 2.7652, p=0.006. (F) The paired- pulse ratio (PPR) for oEPSCs is reduced in Pogz+/- FSINs, U = 2.128, p=0.03. (G) The latency of the first optically-evoked action potential is increased in Pogz+/- FSINs, U = −2.490, p=0.013. (H) The number of action potentials elicited by oEPSPs is non-significantly altered, U = 1.766, p=0.08. In E-H, different hues correspond to specific mice, and squares indicate datapoints from cells that were used for the representative traces shown in A-D. All p-values from Wilcoxon rank sum, WT N = 6 animals, n = 11 cells. Het N = 3 animals, n = 7 cells.

Figure 4—source data 1. Source data for Figure 4.

Figure 4.

Figure 4—figure supplement 1. Intrinsic properties of prefrontal FSIN are not changed in Pogz+/- mice.

Figure 4—figure supplement 1.

(A, B) Representative examples of FSIN responses to current injection in WT (left) or Pogz+/- (right) mice. (C) Membrane resting potential, U = −0.6792, p=0.50. (D) Input resistance, U = −0.7698, p=0.44. (E) Action potential halfwidth, U = −0.724, p=0.47. (F) Maximum firing rate, U = 0.6792, p=0.50. All p-values from Wilcoxon rank sum, WT N = 6 animals, n = 11 cells. Het N = 3 animals, n = 7 cells.