Figure 2.

Overview of the generation and use of cell type-enriched intestinal organoids. Stimulation of organoids with pathogens, specific niche factors, certain drugs, or nutrients results in cell-type enrichment in organoids. These cell type-enriched organoids can be used for a wide variety of different kinds of studies including single-cell scRNA-seq analysis, cell‒cell or cell‒pathogen interaction studies, screens with drugs or small molecules, and studies involving analysis of specific genetic alterations. T, T-cells; NK, natural killer cells; IL, interleukins; IFN, interferons.