Figure 1.

Expression of akr1a1b in Zebrafish Development and in Adult Organs

(A and B) (A) Akr1a1b is ubiquitously expressed in zebrafish development (relatively compared with 24 hpf, n = 4, mean ± SD) and (B) in all analyzed organs of adult zebrafish (relatively compared with heart, n = 5, mean ± SD).

(C) In adult zebrafish kidney, immunohistochemistry revealed high Akr1a1b expression in renal tubules (arrows). Box shows kidney immunostaining with secondary antibody only. Expression of genes in (A and B) was determined by RT-qPCR and normalized to β-actin.

∗∗∗p < 0.001, p value was calculated by one-way ANOVA. Scale bars, 50 μm. See also Figure S1.