Figure 4.

Akr1a1b Knockout Altered Adult Zebrafish Kidneys, but Livers Remained Normal

(A) Periodic acid-Schiff (PAS) staining and (B) electron microscopy (EM) showed deposition of diastase-resistant PAS-positive hyaline droplets, putatively lysosomes (red arrows), within the epithelium of proximal tubules in Δ-23 akr1a1b^-/- kidneys. Akr1a1b^+/+ kidneys only have scattered small droplets. Quantification of 19 akr1a1b^-/- EM images revealed 9 images with “+” and 10 images with “++” PAS-positive hyaline droplets. All 20 akr1a1b^+/+ EM images were scored as “0.”

(C) Adult Δ-23 akr1a1b^-/- livers stained by PAS were unaltered.

Scale bars: 20 μm in (A and C) and 500 nm (B).