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. 2020 Nov 4;23(12):101765. doi: 10.1016/j.isci.2020.101765

Figure 2.

Figure 2

Single Cell Sequencing Reveals Cellular Heterogeneity of the SVF from iWAT

(A) Gene expression heatmap showing the top 5 most differentially expressed (DE) genes (ordered by) used for biological identification of each cluster compared to all other clusters. Genes are represented in rows and ordered by decreasing p value and cell clusters in columns.

(B–D) (B) UMAP plots of iWAT SVF populations from combined data set of control, CL-, and cold-treated mice for 3 days representation, which identified 20 clusters from 6022 cells. UMAP expression plots of representative DE genes displaying expression of known as in (C) adipocyte progenitors' cell markers and in (D) immune cell markers overlaid in red across all populations.

Scale bars represent z-test-normalized gene expression in (A) and gene counts in (C and D). MSCs, mesenchymal stem cells; PreAd, committed pre-adipocytes; Mac1, M1 macrophages; Mac2, M2 macrophages; Mono, monocytes; DC, dendritic cells; pDC, plasmacytoid dendritic cell; CD4T, CD4+ T cells; CD8T, CD8+ T cells; B, B cells; NKs, natural killer cells; HSCs, myeloid progenitor cells.