Figure 3.

CL and Cold Differently Alter the Adipose Resident Immune Compartment

(A) Side-by-side UMAP plots of iWAT SVF populations from control, CL-, or cold-treated mice for 3 days.

(B) Bar chart showing population fold changes in relative abundance of each cluster induced by 3 days of CL or cold treatment compared to vehicle-treated mice.

(C) Proportions of cell populations grouped by the cellular origin.

(D) Quantification of frequency of selected myeloid cell subsets in the iWAT depot after 3 days of CL or cold treatment compared to vehicle-treated mice (n = 6) performed by flow cytometry.

(E) Quantification of frequency of selected lymphoid cell subsets in the iWAT depot after 3 days of CL or cold treatment compared to vehicle-treated mice (n = 6) performed by flow cytometry.

Data are presented as mean ± SEM. p values. n = 5–6 animals in each group; ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.