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. 2020 Nov 5;23(12):101780. doi: 10.1016/j.isci.2020.101780

Figure 7.

Figure 7

TBsRNA-33 Is an Anti-Sense Regulator of Tb927.11.13610, Rhodanese-like Domain-Containing Protein mRNA

(A) TBsRNA-33 interacts with Tb927.11.13610 mRNA. Schematic representation of the potential base-pairing between TBsRNA-33 and Tb927.11.13610 mRNA.

(B) The sequence of the chimeric RNA formed by the interaction between TBsRNA-33 and the Tb927.11.13610 CDS region is indicated based on the PRS interactome library.

(C) Tb927.11.13610 mRNA is not increased upon TBsRNA-33 silencing. Total RNA (20 μg) was prepared from cells before and after TBsRNA-33 silencing, separated on a 1.2% agarose/formaldehyde gel and subjected to Northern analysis using RNA probes, as indicated. 7SL RNA served as a loading control.

(D) Tb927.11.13610 translation is increased upon TBsRNA-33 silencing. Tb927.11.13610 was tagged with N-terminal MYC tag in cells carrying the silencing construct for TBsRNA-33 and were silenced for the indicated number of days. Whole cell lysates from induced (+TET) and uninduced (-TET) cultures were subjected to western analysis with the indicated antibodies (left). (E) The expression level for Tb927.11.13610-MYC is presented as the mean ± S.E.M. Experiments were performed in triplicate (n = 3). Student's t-test was calculated, and the p value is indicated. The expression level of HSP83 was used as a loading control. All densitometry data were calculated by ImageJ (https://imagej.nih.gov/ij/) software.