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. 2020 Nov 19;12:11845–11855. doi: 10.2147/CMAR.S267666

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© 2020 Yang et al.

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miR-1224-5p inhibited the proliferation, migration and invasion of gastric cancer cells via downregulating RSF1. (A) Bioinformatics and luciferase assay clarified that miR-1224-5p can directly binding to the RSF1 3ʹ-UTR region. (B) The miR-1224-5p mimics and luciferase reporter plasmids with wild-type or mutant RSF1 3ʹ-UTR were co-transfected into SNU-16 cells. Dual luciferase reporter gene assay was performed to verify the direct binding relationship between miR-1224-5p and RSF1. (C) SNU-16 was transfected with mimics NC, miR-1224-5p mimics, inhibitor NC, miR-1224-5p inhibitor, and then qRT-PCR was taken to evaluate relative expression levels of miR-1224-5p and RSF1. (D) Cell proliferation was tested by CCK-8 assay. (E) Cell invasion was detected by transwell assay. (F) The number of invaded cells. (G) Cell migration was detected by wound healing assay. (H) The distance of the cell migration detected by wound healing assay. Data are shown as mean ± SD. **P < 0.01.