Immunofluorescent characterization of cell type expression of PI5P4Kα and PI5P4Kβ in the mouse brain
PI5P4Kα (Abgent) does not localize with GFAP in the hippocampal pyramidal cell layer (a) but localizes with Olig2 in corpus callosum (b). PI5P4Kα expression is not evident at the immunofluorescent level in neurons within the hippocampal pyramidal cell layer (c). PI5P4Kβ localizes with NeuN (f) but not Olig2 (d) or GFAP (e) in the hippocampal pyramidal cell layer. Arrowheads identify positive staining for PI5P4Kα and PI5P4Kβ.
CC, corpus callosum; PCL, pyramidal cell layer; SO, stratum oriens; SR, stratum radiatum Scale bars = 30 μm.