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. Author manuscript; available in PMC: 2021 Nov 1.
Published in final edited form as: Microb Pathog. 2020 Aug 21;148:104463. doi: 10.1016/j.micpath.2020.104463

Figure 5. Effect of Lactobacillus supernatant on GBS association with and invasion of dT-HESCs.

Figure 5.

The colonizing GB112 and invasive GB411 strains were added to dT-HESCs at a MOI of 10 for two hours with 10% v/v of supernatant from each Lactobacillus strain; GBS without Lactobacillus supernatant was included for comparison. The percent of associated GBS was calculated for A) GB112 and B) GB411 as was the percent of invaded bacteria for C) GB112 and D) GB411. Percentages were calculated relative to the total number of bacteria in the well and were examined as a percentage of each GBS strain alone to account for variation between trials. Experiments were completed in at least biological triplicates of technical triplicates. Error bars represent standard deviation between biological trials. Significance between GBS alone and with supernatant was determined by an unpaired ANOVA. p < 0.05 *