Fig. 6.

Nephrocytes in crayfish (II): reconstruction images. (a, a′) Reconstruction images of two adjacent nephrocytes (green and purple). Adjacent nephrocytes interdigitated each other. There was no obvious intercellular space between them. (b) Apical view of a single reconstructed nephrocyte. This cell is contained by the epithelioid shown in a, a′. Three primary processes (PP1–PP3), which typically exhibit a rounded shape, were projected from the periphery of the cell body (CB). (c, d) Basal view of reconstructed nephrocytes. Two adjacent reconstructed nephrocytes are separated. The purple cell shows the basal surface of primary processes (d). The green cell shows the basal surface of cell body, on which the primary processes of the adjacent purple cell were imprinted (dotted line in c). From the cell body and primary processes, numerous foot processes (whitish-green in c, whitish-purple in d) protruded via ridge-like prominences (yellowish-green in c, yellowish-purple in d). (d′) Apical view of the purple nephrocyte shown in d. Scale bars: 2 μm in a, a′; 500 nm in b–d. The reconstructed nephrocytes (b–d) are also shown in Supplementary Movies S5 and S6