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. 2020 Nov 16;382(3):609–625. doi: 10.1007/s00441-020-03313-7

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© The Author(s) 2020

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Fig. 8 — Nephrocytes in prawn (I): FIB-SEM sectional images. (a) Schematic drawing of multiple nephrocytes (N) forming a large epithelial sac with an obvious lumen (L). The sac is partially altered into flat cells (F, green cells). There is no cytoplasmic continuity between nephrocytes. (b) Semi-thin resin-section. The lumen of the epithelial sac is clearly visible with light microscopy. (c–f) FIB-SEM sectional images. (c, f) The connecting part (arrows) of nephrocytes (purple) and flat cells (green). Flat cells were in contact with the internal wall of the efferent branchial vessel (EBV). (d) Nephrocytes contained huge lysosomes (Ly). The cell bodies of adjacent nephrocytes were connected via spotty intercellular junctions (arrows in d) that are structurally different from the slit diaphragm between foot processes (arrowheads in e). Intercellular space (asterisks in d, f) was widely opened between nephrocytes. BM, basement membrane. Scale bars, 5 μm in b; 1 μm in c, d, f; 100 nm in e