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. 2020 Nov 24;6:87. doi: 10.1038/s41421-020-00211-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a QRT-PCR of lncRNAs in bone marrow-derived macrophages (BMDMs) after exposed to butyrate (200 μM). R. E relative expression. b QRT-PCR of lncLy6C in BMDMs after exposed to ascetic acid (200 μM), propionic acid (200 μM, and butyrate (200 μM). WT control vehicle, R. E relative expression. c Northern blot of lncLy6C in BMDMs after exposed to butyrate. WT control vehicle. d QRT-PCR of lncLy6C in BMDMs after exposed to different concentration of butyrate. R. E relative expression. e QRT-PCR of lncLy6C in BMDMs after exposed to butyrate (200 μM) at different time points. R. E relative expression. f FISH of lncLy6C in BMDMs after exposed to butyrate (200 μM) at different time points. Nuclei were stained with DAPI (blue); green, lncLy6C. Scale bar, 2.5 μM. g QRT-PCR of lncLy6C in spleen, B cell, CD4, CD8, dendritic cells (DC), myeloid-derived suppressive cells (MDSC), and macrophages sorted from spleen by flow cytometry. R. E relative expression. h FISH of lncLy6C and immunostaining of F4/80⁺, CD11c⁺, MDSC (ly6c⁺), CD4⁺, CD8⁺, and B (CD19⁺) cells in spleen. Nuclei were stained with DAPI (blue); green, lncLy6C. Scale bar, 40 μM. i QRT-PCR of lncLy6C in human CD14⁺ monocytes after exposed to different concentration of butyrate. R. E relative expression. j QRT-PCR of lncLy6C in human CD14⁺ monocytes after exposed to butyrate (200 μM) at different time points. R. E relative expression. k Flow cytometry of CD115⁺Ly6C^high and CD115⁺Ly6C^low cells in lncLy6C microRNA (kdLNC) or lncLy6C (oeLNC) lentivirus transfected BMCs after culturing for 4 days in the presence of GM-CSF. KdNC control microRNA lentiviruses, oeNC control lentiviruses. l Comparison f Ly6C^high and Ly6C^low cells in lncLy6C microRNA (kdLNC) or lncLy6C (oeLNC) lentivirus transfected BMCs and control microRNA (kdNC) or control lentivirus (oeNC) transfected BMCs, two-sided Student’s t test in a, b, d, e, g, i, j, and l; data for all panels are a representative from two to three experiments.