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. 2020 Nov 23;11(11):1004. doi: 10.1038/s41419-020-03204-3

Fig. 6. CRH induces mitochondrial fission.

Fig. 6

A Graphical representation of mitochondrial dynamic: fusion and fission with relative proteins (OMM = outer mitochondrial membrane; IMM = inner mitochondrial membrane). Primary hippocampal neurons at DIV14 were treated with CRH 100 nM for 0.5 h and with CRH 0.5 h + CRHR1 Blocker NBI30775 100 nM; Western Blot analysis and quantification of B the fission proteins DRP1S616, C FIS1 and the fusion markers D OPA1, E MFN1 and F MFN2. G Summary of the western blot results. Experiments were performed in N = 3 independent replicates at DIV14. Data are displayed as Mean ± SEM; one-way ANOVA and Bonferroni’s post hoc comparison test were performed; data non normally distributed were analyzed by Kruskal–Wallis non parametric test followed by Uncorrected Dunn’s multiple comparison test. (*p < 0.05, **p < 0.005, ***p < 0.0005, ****p < 0.0001).