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. 2020 Nov 23;10:20363. doi: 10.1038/s41598-020-76702-1

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Schematic representation of mechanisms for the selection and expansion of CD8 + T cells by OC-expanded supercharged NK cells. Freshly purified NK cells with 1–3% contaminated T cells (CD4 + and CD8 + T cells) were treated and co-cultured with OCs as described in Materials and Methods (left panel). After days 12–25 of co-culture, the OC-expanded cells express approx. 20% T cells majority of which are CD8 + T cells (right panel). The expansion of CD8 + T cells is likely due to the lysis of CD4 + T cells by the super-charged NK cells.