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. 2020 Nov 10;11:582214. doi: 10.3389/fimmu.2020.582214

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Copyright © 2020 Graham, Yoachim and Gould

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Disruption of ERα abrogates Sle1b-induced T_fh cell hyperactivation in female mice. (A) Dot plots show the percentage of splenocytes in female B6 (N=23), female B6.Sle1b (N =30), male B6 (N=19), and male B6.Sle1b (N= 32) mice that were CD4⁺CXCR5^hiPD-1^hi follicular helper T (T_fh) cells. (B) Representative contour plots show the frequency of CD4⁺CXCR5^hiPD-1^hi T_fh cells in female and male B6 and B6.Sle1b mice. (C) Dot plots show the percentage of splenocytes in female B6.ERα^+/+ (N=23), B6.ERα^-/- (N=10), B6.Sle1b.ERα^+/+ (N=30), and B6.Sle1b.ERα^-/- (N=13) mice that were CD4⁺CXCR5^hiPD-1^hi T_fh cells. (D) Representative contour plots show the frequency of CD4⁺CXCR5^hiPD-1^hi T_fh cells in female B6.ERα^+/+, B6.ERα^-/-, B6.Sle1b.ERα^+/+, and B6.Sle1b.ERα^-/- mice. (E) Dot plots show the percentage of splenocytes in male B6.ERα^+/+ (N=19), B6.ERα^-/- (N=5), B6.Sle1b.ERα^+/+(N=32), and B6.Sle1b.ERα^-/- (N=11) mice that were CD4⁺CXCR5^hiPD-1^hi T_fh cells. (F) Representative contour plots show the frequency of CD4⁺CXCR5^hiPD-1^hi T_fh cells in male B6.ERα^+/+, B6.ERα^-/-, B6.Sle1b.ERα^+/+, and B6.Sle1b.ERα^-/- mice. Splenocytes were collected from mice that were 5–6 months of age. In (A, C, E), the longer horizontal bar in each panel denotes the mean for each group, and the shorter black bars indicate the standard error of the mean. The ** indicates p ≤ 0.01.