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. 2020 Nov 10;11:582214. doi: 10.3389/fimmu.2020.582214

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Copyright © 2020 Graham, Yoachim and Gould

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Disruption of ERα attenuates formation of spontaneous germinal center formation. (A) Dot plots show the percentage of splenocytes that were B220⁺CD95⁺PNA^hi germinal center B cells in female B6 (N=23), female B6.Sle1b (N =30), male B6 (N=19), and male B6.Sle1b (N= 32) mice. (B) Representative contour plots show the frequency of B220⁺CD95⁺PNA^hi B cells in female and male B6 and B6.Sle1b mice. (C) Dot plots show the percentage of splenocytes that were B220⁺CD95⁺PNA^hi germinal center B cells in female B6.ERα^+/+ (N=23), B6.ERα^-/- (N=10), B6.Sle1b.ERα^+/+ (N=30), and B6.Sle1b.ERα^-/- (N=13) mice. (D) Representative contour plots show the frequency of B220⁺ CD95⁺PNA^hi B cells in female B6.ERα^+/+, B6.ERα^-/-, B6.Sle1b.ERα^+/+, and B6.Sle1b.ERα^-/- mice. (E) Dot plots show the percentage of splenocytes that were B220⁺CD95⁺PNA^hi germinal center B cells in male B6.ERα^+/+ (N=19), B6.ERα^-/- (N=7), B6.Sle1b.ERα^+/+ (N=32), and B6.Sle1b.ERα^-/- (N=11) mice. (F) Representative contour plots show the frequency of B220⁺CD95⁺PNA^hi B cells in male B6.ERα^+/+, B6.ERα^-/-, B6.Sle1b.ERα^+/+, and B6.Sle1b.ERα^-/- mice. (G) Representative images of PNA staining in sections from the spleen of female B6.ERα^+/+ (N=3), B6.ERα^-/- (N=3), B6.Sle1b.ERα^+/+ (N=7), and B6.Sle1b.ERα^-/- (N=7) mice are shown. (H) Representative images of PNA staining in sections from the spleen of male B6.ERα^+/+(N=3), B6.ERα^-/- (N=3), B6.Sle1b.ERα^+/+ (N=7), and B6.Sle1b.ERα^-/- (N=3) mice are shown. Spleens and splenocytes were collected from mice that were 5–6 months of age. The longer horizontal bar in each panel denotes the mean for each group, and the shorter black bars indicate the standard error of the mean. The * indicates p ≤ 0.05, and the ** indicates p ≤ 0.01.