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. 2020 Nov 23;7:412. doi: 10.1038/s41597-020-00744-3

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© The Author(s) 2020

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Fig. 2 — Statistics of the primary human T-cell spectral library. (a) Statistics of individual spectral libraries used in the generation of human T-cell spectral library. (b) Protein, peptide and peptide spectral matches (PSMs) of individual libraries as function of protein FDR. (c) Venn diagram depicting the protein overlaps between different spectral libraries. (d) Frequency distribution of peptides of different lengths in human T-cell spectral library. (e) Frequency distribution of proteins with different peptide numbers in human T-cell spectral library. (f) First 30 significant immune related canonical pathways represented by the T-cell spectral library (Core analysis, Ingenuity pathway analysis, Qiagen Bioinformatics, USA) (g) Protein coverage of the human T-cell spectral library for T-cell receptor (TCR) signaling pathways. Proteins highlighted in pink are included in human T-cell spectral library. (SpLib_1 - in vitro activated human T-cell spectral library, SpLib_2, 3 and 4 - ex vivo human T-cell spectral libraries).