Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Nov 23;11(11):1008. doi: 10.1038/s41419-020-03169-3

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 1 — A Scanning of exosomes isolated from culture media of MCs using electron microscopy. B Scanning of exosomes isolated from human serum using electron microscopy. C CD63, Alix, and Tsg101 protein expression in culture media of MCs was detected using western blotting analysis. D CD63, Alix, and Tsg101 protein expression in human serum was evaluated using western blotting analysis. E The expression level of circ_DLGAP4 was detected in exosomes isolated from culture media of MCs using qRT-PCR. MCs were treated with NG (5.5 mM) or HG (25 mM) for 24 h. F The expression level of circ_DLGAP4 was tested in exosomes isolated from human serum samples of T2D and DKD patients. G The expression level of circ_DLGAP4 was detected in human serum samples of T2D patients without DKD (n = 34) or with DKD (n = 44). H Expression levels of circ_DLGAP4 was detected in DKD with microalbuminuria (n = 12) or with macroalbuminuria (n = 32). I The expression level of circ_DLGAP4 were detected in DKD with low eGFR (n = 14) or high eGFR (n = 30). J HE staining in NC and DKD rat model. n = 8 in each group. K Blood glucose was detected in NC and DKD rat model. L Western blotting analysis of p-cadherin and Mcp-1 in NC and DKD rat model. M The expression level of circ_DLGAP4 was detected in NC and DKD rat model. Three independent experiments were conducted. Error bars stand for the mean ± SD of at least triplicate experiments; **P < 0.01.