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. 2020 Nov 23;11(11):1008. doi: 10.1038/s41419-020-03169-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — A The expression level of circ_DLGAP4 was detected in exosomes isolated from MCs culture media treated with NC or HG. B Validation of circ_DLGAP4 using reverse transcription PCR (RT-PCR) analysis. C The expression level of circ_DLGAP4 was detected in PBS, exosomes, and exosomes-circRNA del. Exosomes were collected after si-circRNA transfection for 48 h in MCs in exosomes-circRNA del group. D, E EdU and CCK-8 were carried out to test MCs proliferation and viability. F Flow cytometric assay was used to evaluate cell cycle progression in MCs. G Western blotting was used to test p-53, p-cadherin, and Mcp-1 protein expression. H, I Efficiency of LV-circ_DLGAP4 and sh-circ_DLGAP4 in MCs were detected by qPCR. J, K EdU assay was carried out to test cell proliferation. L, M Results of CCK-8 assays. N, O Cell cycle analysis using flow cytometric assay. P, Q Western blotting assay was used to assess p-53, p-cadherin, and Mcp-1 protein expression in MCs. Three independent experiments were conducted. Error bars stand for the mean ± SD of at least triplicate experiments; *P < 0.05, **P < 0.01.