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. 2020 Nov 23;11(11):1008. doi: 10.1038/s41419-020-03169-3

Fig. 4. miR-143 suppressed proliferation and fibrosis of MCs by targeting ERBB3.

Fig. 4

A Five genes were screened in MCs transfected with mimic-miR-143. B Five genes were screened in MCs transfected with inhibitor-miR-143. C, D The protein expression level of ERBB3 in MCs transfected by mimic-miR-143 or inhibitor-miR-143. E The direct binding sites between miR-143 and ERBB3 were presented. F Luciferase reporter assay was performed to confirm the direct binding relationship between miR-143 and ERBB3. G, H Proliferating MCs were labeled with EdU after transfection with mimic-miR-143 or inhibitor-miR-143. I, J Proliferating MCs were labeled with CCK-8 after transfection with mimic-miR-143 or inhibitor-miR-143. K, L The protein expression level of p-53, p-cadherin, and Mcp-1 in MCs transfected with mimic-miR-143 or inhibitor-miR-143. M, N Efficiency of pcDNA3.1-ERBB3 were confirmed by qPCR and western blotting. MCs were transfected with pcDKDA3.1-ERBB3 or vector. O CCK-8 assay was carried out to evaluate cell proliferation. MCs were transfected with pcDNA3.1-ERBB3 or vector. P p-53, p-cadherin, and Mcp-1 protein expression in MCs transfected with pcDNA3.1-ERBB3 or vector. Q The growth of MCs was detected after transfected by mimic-miR-143 or pcDNA3.1-ERBB3. R p-53, p-cadherin, and Mcp-1 protein expression in MCs transfected by mimic-miR-143 or pcDNA3.1-ERBB3. Three independent experiments were conducted. Error bars stand for the mean ± SD of at least triplicate experiments; *P < 0.05, **P < 0.01.