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. 2020 Nov 23;11(11):1008. doi: 10.1038/s41419-020-03169-3

Fig. 5. circ_DLGAP4 promoted proliferation and fibrosis of MCs by regulating ERBB3/p-NF-κB/MMP-2 axis and sponging miR-143.

Fig. 5

A, B The mRNA expression level of ERBB3 in MCs infected with LV-circ_DLGAP4 or sh-circ_DLGAP4. C, D The protein expression level of ERBB3 in MCs infected with LV-circ_DLGAP4 or sh-circ_DLGAP4. E The mutant-type reporter gene (Mut-DLGAP4 reporter) and wild-type reporter gene (Wt-DLGAP4 reporter) were established. MCs were co-transfected with LV-circ_DLGAP4 or sh-circ_DLGAP4 with Mut-DLGAP4 reporter or Wt-DLGAP4 reporter. F, G The expression level of NF-κB/p-NF-κB was measured by western blotting assay. MCs were transfected by LV-circ_DLGAP4 or sh-circ_DLGAP4. H, I The mRNA expression level of MMP-2 in MCs. J, K The protein expression level of MMP-2 in MCs. L The expression level of p-NF-κB and MMP-2 in MCs treated with LV-circ_DLGAP4 or ERBB3 siRNA. M Proliferation of MCs transfected with LV-circ_DLGAP4 or mimic-miR-143. N The protein expression level of p-53, p-cadherin, and Mcp-1 in MCs treated with LV-circ_DLGAP4 or mimic-miR-143. Three independent experiments were conducted. Error bars stand for the mean ± SD of at least triplicate experiments; **P < 0.01, ***P < 0.001.