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. 2020 Nov 23;11(11):1008. doi: 10.1038/s41419-020-03169-3

Fig. 6. The effects of exo-circ_DLGAP4 on miR-143 and ERBB3/p-NF-κB/MMP-2 axis in MCs.

Fig. 6

A The expression level of miR-143 was detected in MCs treated with NC or exosomes. B The protein expression level of ERBB3/p-NF-κB/MMP-2 axis was detected in MCs treated with NC or exosomes. C The mutant-type reporter gene (Mut-ERBB3 reporter) and wild-type reporter gene (Wt-ERBB3 reporter) were established. MCs were co-transfected with NC or exosome with Mut-ERBB3 reporter or Wt-ERBB3 reporter. Luciferase reporter assay was performed to confirm the relationship between exosomes and ERBB3. D–F The protein expression level of p-NF-κB and MMP-2 was detected in Wt-ERBB3 group and Mut-ERBB3 group. G, H The protein expression level of ERBB3 in MCs treated with exosomes or mimic-miR-143. I The expression level of miR-143 was detected in MCs treated with exosomes or exo-circRNA del. J The protein expression level of ERBB3/p-NF-κB/MMP-2 axis was detected in MCs treated with exosomes or exo-circRNA del. K The Mut-ERBB3 and Mt- ERBB3 reporter were established. MCs transfected with Mut-ERBB3 reporter or Wt-ERBB3 reporter were treated by exosomes or exo-circRNA del. Luciferase reporter assay was performed to confirm the relationship between exo-circRNA del and ERBB3. L The mRNA expression level of MMP-2 was detected in Wt-ERBB3 group. M The protein expression level of MMP-2 and p-NF-κB was detected in Wt-ERBB3 group. Three independent experiments were conducted. Error bars stand for the mean ± SD of at least triplicate experiments; **P < 0.01.