Table 1.

Kinetic parameters for prephenate dehydratase activity of CDT variants related to this study^a.

Protein	KM (μM)	kcat (s−1)	kcat/KM (M−1 s−1)
AncCDT-140	ND	ND	ND
AncCDT-3/P18840	1830 ± 190	(1.04 ± 0.07) × 10−2	5.67 ± 0.70
AncCDT-3/L18840	294 ± 27	(4.58 ± 0.30) × 10−2	155 ± 18
AncCDT-5	277 ± 4	4.1 ± 0.2	(1.5 ± 0.1) × 104
PaCDT40	18.7 ± 2.9	18.4 ± 0.7	(9.8 ± 1.6) × 105

ND not detected.

^aThe results are reported as mean ± standard error for K_M and k_cat. Errors for k_cat/K_M were obtained by propagation. Source data are provided as a Source Data file.