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. 2020 Nov 10;10:557730. doi: 10.3389/fonc.2020.557730

Figure 1.

Figure 1

THBS2 was aberrantly upregulated and displayed a tumor-promoting role in CRC. (A) The mRNA expression of THBS2 in CRC and adjacent non-cancerous tissues, as analyzed from the TCGA dataset. (B) The mRNA expression of THBS2 in CRC and adjacent non-cancerous tissues, as analyzed from the GDS4382 dataset. (C) The mRNA expression of THBS2 in CRC and adjacent non-cancerous tissues, as analyzed from the GSE6988 dataset. (D) The mRNA expression of THBS2 in CRC and adjacent non-cancerous tissues, as analyzed from the GSE35834 dataset. (E) The mRNA expression of THNB2 in six CRC cell lines. (F) The viability of LoVo cells transfected with sh-THBS2 or sh-control, as analyzed with CCK-8 assay. (G) The viability of RKO cells transfected with sh-THBS2 or sh-control, as analyzed with CCK-8 assay. (H) The viability of SW620 cells transfected with Vector or THBS2-OV, as analyzed with CCK-8 assay. (I) Establishing an orthotopic tumor model by injecting tumor cells with luciferase into the cecum of BALB/c nude mice and imaging by CT. (J) Vivo imaging of orthotopic tumor by injecting sh-THBS2 or sh-control LoVo cell and Vector or THBS2-OV SW620 cells in the cecum (n = 6 in every group). (K) Tumor burden and Tumor volume in the cecum by injecting sh-THBS2 or sh-control LoVo cell (n = 6 in every group). (L) Tumor burden and Tumor volume in the cecum by injecting Vector or THBS2-OV SW620 cells (n = 6 in every group). (M) Expression of Ki-67 in the orthotopic tumor tissues. All experiments were performed in triplicate. Measurement data are presented as the mean ± SD. Student’s t-test was used for statistical analysis; *p < 0.05, **p < 0.01, ***p < 0.001.