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. 2020 Nov 10;10:557730. doi: 10.3389/fonc.2020.557730

Figure 4.

Figure 4

THBS2/TLR4 axis increased HIF-1α expression in CRC cells. (A) Protein expression of HIF-1α of LoVo cells in the sh-NC and sh-THBS2 group and SW620 cells in the Vector and THBS2-OV group. (B) Protein expression of HIF-1α of LoVo and RKO cells in the sh-NC and sh-THBS2 groups. (C) Protein expression of HIF-1α of SW620 and SW480 cells in the Vector, THBS2-OV, THBS2-OV+siTLR4, and THBS2-OV+TAK-242 group. (D) Glucose uptake of SW620 or SW480 cells in the Vector, THBS2-OV, THBS2-OV+HIF1α group. (E) Lactic acid formation of SW620 or SW480 cells in the Vector, THBS2-OV, THBS2-OV+HIF1α group. (F) mRNA expression of GLUT1, HK2, ALDOA, PKM2, and LDHA of SW620 and SW480 cells in the Vector, THBS2-OV, and THBS2-OV+siHIF-1α group. (G) The viability of SW620 or SW480 cells in the Vector, THBS2-OV, and THBS2-OV+siHIF-1α group, as analyzed with CCK-8 assay. Student’s t-test was used for statistical analysis. ns. represents no statistical difference; *p < 0.05, **p < 0.01.