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. 2020 Nov 17;15:2991–3001. doi: 10.2147/COPD.S280614

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© 2020 Zhang et al.

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Screening and validation of differentially expressed miRNAs. Heat maps of differentially expressed miRNAs in COPD patients vs those in normal individuals, based on the GSE38974 dataset and miR-486-5p expression in the GSE38974 dataset (A). Heat maps of differentially expressed miRNAs in smokers vs those in nonsmokers, based on the GSE53519 dataset and miR-486-5p expression in the GSE53519 dataset (B). miR-486-5p expression in alveolar macrophages of COPD patients (n=36) and normal individuals (n=14), determined using qPCR (C). miR-486-5p expression in peripheral monocytes in smokers (n=42) vs that in the peripheral monocytes in nonsmokers (n=33) and in COPD I–II (n=30) vs COPD III–IV (n=23), determined using qPCR (D). *P < 0.05; **P < 0.01; ***P < 0.001.