Figure 4.

Constant light does not affect the size or severity of atherosclerotic lesions. APOE*3-Leiden.CETP mice fed a Western-type diet were exposed to LD or LL (n = 18/group) for 14 weeks, after which hearts were isolated and the valve area of the aortic root was stained with hematoxylin-phloxine-saffron. (A) Mean atherosclerotic lesion area was determined from 4 cross sections of the aortic root (B), and lesion severity (mild, type I-III vs severe, and type IV-V) was scored (C). Data are expressed as individual values, including the means ± SEM. Significance was tested by 2-tailed unpaired Student t test, of which the test statistics are included in Supplementary Table S2.