Fig. 1.

Bioengineered let-7c exerts a strong synergism with 5-FU but none or minimal with DOX or SFB in the inhibition of HCC cell viability. (A) Huh7 and Hep3B cells transfected with 1 or 2.5 nM of bioengineered let-7c or control MSA were treated with various concentrations of 5-FU, DOX, or SFB. Inhibition of cell viability was measured by ATP luminometric assay, and the data were fit to an inhibitory dose-response model. Vehicle control was set as 0% inhibition. (B) The Chou-Talalay plots [log(CI) vs. Fa] revealed a strong synergism for let-7c plus 5-FU combination treatment.