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. 2020 Dec;48(12):1257–1263. doi: 10.1124/dmd.120.000207

Fig. 3.

Fig. 3.

Let-7c suppresses TS expression in Huh7 cells, whereas 5-FU inhibits 5-FU. Expression of TS and other related proteins in Huh7 cells was analyzed by immunoblot at 24, 48, and 72 hours post-treatment with 5-FU (1 µM) and let-7c (10 nM) alone or in combination. Cotargeting TS by 5-FU and let-7c is manifested by the appearance of 5-FU metabolite FdUMP-bound (inhibited, top band) TS in cells treated with 5-FU and significant reduction of unbound and total TS protein levels in cells treated with let-7c. Protein levels of known let-7c–targeted genes RAS, ARID3B, LIN28B, and cellular myelocytomatosis oncogene (c-MYC) were also significantly reduced by bioengineered let-7c. Protein expression level was normalized to corresponding total protein (Tol. prot.) level, and differences between each treatment groups were compared by one-way ANOVA with Bonferroni’s post hoc tests. Values are mean ± S.D. (N = 3/group). P < 0.05, compared with vehicle (a), 5-FU alone (b), or let-7c alone (c).