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. 2020 Nov 23;11(11):1003. doi: 10.1038/s41419-020-03209-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Nuclear morphology was quantified through high-content imaging in MCA205 (a–d), 71-7 (e–h), MUCC (i–l) cells, 48 h after irradiation. Cells were stained with Hoechst and propidium iodide after permeabilization to allow segmentation of the nucleus and cytoplasm, respectively. Nuclear area was measured in MCA205 (a), 71-7 (e), and MUCC (i). The roundness of the nucleus was also assessed after irradiation in MCA205 (b), 71-7 (f), and MUCC (j). Percentage of cells with micronuclei is shown for MCA205 (c), 71-7 (g), and MUCC (k) cells. The area of the cells was also measured following irradiation in MCA205 (d), 71-7 (h), and MUCC (l) cells. m Representative picture of micronuclei segmentation. n Representative picture of segmentation mask for the assessment of the nuclear shape.