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. 2020 Nov 23;11(11):1003. doi: 10.1038/s41419-020-03209-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — Senescence induction was measured 72 h following irradiation at the indicated doses by flow cytometry with C12FDG, a β-galactosidase substrate emitting fluorescence upon its cleavage. Co-staining with Annexin V-APC and Sytox Blue was performed to indicate apoptosis and membrane permeabilization, respectively, in CT26 (a–d), MCA205 (e–h), and 71-7 (i–l) cells. Representative flow cytometry histograms are shown for C12FDG (SA-β-Gal) staining in CT26 (a), MCA205 (e), and 71-7 (i). Percentage of cells positive for C12FDG (SA-β-Gal) and/or Sytox Blue is shown for CT26 (b), MCA205 (f), and 71-7 (j) cells. Percentage of cells positive for Annexin V-APC and/or Sytox Blue is shown for CT26 (c), MCA205 (g), and 71-7 (k). Percentage of cells positive for C12FDG (SA-β-Gal) and/or Annexin V-APC is shown for CT26 (d), MCA205 (h), and 71-7 (l). Means ± SEM are shown (n = 2). SA-β-Gal senescence-associated β-Galactosidase.