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. 2020 Nov 23;11(11):1003. doi: 10.1038/s41419-020-03209-y

Fig. 6. Ionizing radiation induces lipid peroxidation and an iron-dependent type of cell death.

Fig. 6

a–c Measurement of lipid peroxidation using C11-BODIPY probe and cell death using Sytox Blue in CT26 (a), MCA205 (b), and 71-7 (c) cells 24 h after irradiation with 20 or 50 Gy. Increase in lipid peroxidation can be seen in the first left quadrant by the shift of C11-BODIPY fluorescence measured after 20 Gy (blue) or 50 Gy (red) X-rays. When indicated, the cells were pre-treated with iron chelators CPX and DFO, or with antioxidants vitamin E and Fer-1 1 h before irradiation. Quantification of cell death is shown as percentage for CT26 (a), MCA205 (b), and 71-7 (c). Means ± SEM are shown (n = 2–3). A two-way ANOVA was performed with a Tukey’s multiple comparisons test. Asterisks (*) show the comparison to 20 Gy Methuosis. *p ≤ 0.05, **p ≤ 0.01.