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. 2020 Nov 23;10:20405. doi: 10.1038/s41598-020-77175-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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hCB1R-dependent recruitment of βarrestin2. hCB1R-dependent recruitment of βarrestin2 was quantified in PathHunter CHO cells stably expressing hCB1R and treated with 0.1 nM–10 µM (a) THC-like compounds; (b) CBD-like compounds; or (c) CBG, or CBC for 90 min. Data were fit to a variable slope (4 parameter) non-linear regression in GraphPad (v. 8). n ≥ 6 independent experiments performed in triplicate. E_Max refers to the top of the concentration–response curve. Data are expressed as mean ± SEM. EC₅₀ and E_max are reported in Table 3.