Figure 2.

DSF screens of FDA-approved drugs and drug-like compounds for SARS-CoV-2 Mac1 ligands. T_m values calculated by fitting melting curves to eq 1 (open circles) obtained for Mac1 in the presence of each compound in (A) the NCI library, (B) the NIHcc, and (C,D) the LOPAC¹²⁸⁰. Assays yielding a “typical” melting curve, as defined by the TSA-CRAFT algorithm, are noted (filled circles). (E) Selected hit compounds separated based on chemotype: nucleotides, steroids, β-lactam antibiotics, and benzimidazoles.