Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Nov 23;8(2):e001478. doi: 10.1136/jitc-2020-001478

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© Author(s) (or their employer(s)) 2020. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.

This is an open access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited, appropriate credit is given, any changes made indicated, and the use is non-commercial. See http://creativecommons.org/licenses/by-nc/4.0/.

PMC Copyright notice

Characterization of Treg within T-cell cultures. (A) Regulatory T cells (Treg) was investigated by intracellular staining for FoxP3 on the CD4⁺CD25^HighCD127⁻ population before and after the rapid expansion protocol (REP) (p=0.2921). (B) Median fold expansion in Treg low and in Treg high culture. There was no significant difference (p=0.5920) (C) Percentage of Treg before and after REP according to T cell immunoglobulin mucin domain-containing protein 3 (TIM-3) expression on CD8 T cells. (D) Glycoprotein-A repetitions predominant (GARP) expression (%) on Treg within peripheral blood mononuclear cells isolated from healthy donors, patients with metastatic colorectal cancer (mCRC) and match Pre-REP T cells. Plots are representative of at least five experiments. (E, F) Kaplan-Meier curve for relapse free survival () according to the percentage of (E) Treg and (F) CD8⁺TIM-3⁺ T cells (low vs high) in Pre-REP T cell cultures following resection of liver metastases from patients with mCRC. Log-rank test was used for the comparison of survival curves. Mann-Whitney tests: *p<0.05, ns, not significant; PBMC, peripheral blood mononuclear cells; TIL, tumor-infiltrating lymphocytes.