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. 2020 Nov 23;19:213. doi: 10.1186/s12934-020-01466-2

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Relative gene expression of metastasis-related genes in colon cancer cells treated with Lactobacillus CFS. a Gene expression in Caco-2 cells; b Gene expression in HT-29 cells. Real-time PCR was carried out to assess changes in MMP2, MMP9, and VEGFA gene expression in colon cancer cells treated with Lactobacillus CFS (untreated group is the negative control and the 5-FU treatment group is the positive control; error bars represent mean of each group ± SD of three independent experiments; a–d, e–g, A–C, different letters in bars mean P < 0.05 (significant differences) by One-way ANOVA in the same group)