Figure 2.

Increased CD4 expression in CD4^hiCD38^hi cells is modulated at the mRNA level. (a) Experimental plan for cell sorting, stimulation and gene expression analysis. (b) Gene expression assessment by qPCR of FACS‐sorted CD4^norm (norm) and CD4^hiCD38^hi (high) T cells from patients with acute P. falciparum malaria (n = 6, log₂‐normalised data, paired t‐test). (c) P. falciparum antigen‐specific responses measured in FACS‐sorted CD4^norm (blue squares) and CD4^hiCD38^hi (red circles) T cells from patients with acute P. falciparum malaria (Pf inf’) or healthy Sabah controls (HC) by qPCR after 24‐h co‐incubation with extract made with P. falciparum‐infected RBC (pRBC) or normal RBC (nRBC) (n = 3 Pf inf’ and n = 3 HC, log₂‐normalised data, paired t‐test).