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. 2020 Nov 23;40(11):BSR20201858. doi: 10.1042/BSR20201858

Figure 2. Effects of miR-19b-3p derived from exosomes on recipient human esophageal cancer cells (EC9706).

Figure 2

(A) Identification of exosomes by transmission electron microscope. (B) Western blot was used to detect surface markers of exosomes. (C) RT-qPCR was used to detect miR-19b-3p expression in mimic control (donor cell), miR-19b-3p (donor cell), mimic control (donor exo), miR-19b-3p (donor exo), Mimic control (recipient), miR-19b-3p (recipient) group. (D) Fluorescence microscope was used to observe the transmission of cy3-miR-19b-3p between EC9706 cells through exosomes. U6 was used as the internal reference gene of miRNA. Results were expressed as the mean ± SD; RT-qPCR, reverse transcription real-time quantitative polymerase chain reaction; ∧∧∧P<0.001 vs. MC; MC, mimic control, M, miR-19b-3p mimic.